1-(3-Hydroxyalk-1-yl)-5-(carboxyalkyl)hydantoin derivatives

ABSTRACT

A compound of formula (I): ##STR1## wherein: X is O or S; 
     n is 1 to 8; 
     R 1  is hydrogen, or CO 2  R 1  represents an ester group in which the R 1  moiety contains from 1-12 carbon atoms; 
     R 2  is hydrogen, C 1-4  alkyl, or phenyl; 
     R 3  is hydroxy or protected hydroxy, 
     R 4  is hydrogen, C 1-9  alkyl, C 3-8  cycloalkyl, C 3-8  cyclo alkyl-C 1-6  alkyl, phenyl, phenyl C 1-6  alkyl, naphthyl, naphthyl-C 1-6  -alkyl, any of which phenyl or naphthyl moieties may be substituted by one or more halogen, trifluoromethyl, C 1-6  alkyl, hydroxy, C 1-6  alkoxy, phenyl C 1-6  alkoxy or nitro groups; or 
     R 2  and R 4  taken with the carbon atom to which they are joined represent a C 5-8  cycloalkyl group; 
     R 5  is C 1-6  alkyl, C 1-6  alkyl substituted by a nitro, hydroxy, C 1-6  alkoxy, CO 2  A, (CO 2  A) 2 , CN or halogen group, C 5-8  cycloalkyl, phenyl, phenyl, phenyl-C 1-6  alkyl, phenyl-C 3-6  cycloalkyl, any of which phenyl moieties may be substituted by one or more halogen, trifluoromethyl, C 1-6  alkyl, C 1-6  alkoxy or nitro groups; or a group CO 2  A; in R 5  when present A is hydrogen or CO 2  A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof; has useful pharmacological activity.

CROSS-REFERENCE TO RELATED APPLICATION

This is a continuation-in-part of Ser. No. 858,562 filed Dec. 8, 1977 now abandoned.

This invention relates to novel compounds having pharmacological activity, to a process for their preparation, to intermediates useful in that process and to pharmaceutical compositions containing them.

Offenlegungsschrift No. 2323193 discloses that pyrazolidine derivatives of the formula (I)': ##STR2## wherein A is CH═CH or C.tbd.C; R is H, an alkali metal, an amine salt, or an ≯12C hydrocarbon or chlorhydrocarbon residue; m is 0 or 1; n is 0-6; p is 0-6; and Y and Z are O or H₂ except that Y and Z are not both O; have similar biological properties to the prostaglandins or are antagonists of prostaglandins.

French Patent Application No. 2258376 discloses that 10-aza prostaglandins of formula (II)": ##STR3## wherein R═H or lower alkyl; R' and R"═CH₃ or C₂ H₅ ; R^(o) ═H or lower alkyl; Y═--CH₂ CH₂ --, or --CH═CH--; Z═--CO or --CH(˜OH)--; are useful in the treatment of blood pressure and gastro-intestinal disorders, and in the preparation for confinement.

Belgian Patent No. 835989 discloses that compounds of the formula (III)": ##STR4## wherein: X is CO, protected CO, CROH in which R is hydrogen or C₁₋₄ alkyl and in which the OH moiety may be protected; Y is CH₂ CH₂ or CH═CH; Z is CO or CH₂ ; n is 1 to 8; m is 1, 2 or 3; R₁ is hydrogen, CH₂ OH, CH₂ OH in which the OH moiety is protected, CO₂ W wherein W is hydrogen or CO₂ W represents an ester group in which the ester moiety contains from 1 to 12 carbon atoms, or CONH₂ ; R₂ is hydrogen, C₁₋₄ alkyl, or taken together with R₃ and the carbon atom to which it is attached represents a carbonyl group; R₃ is hydrogen, hydroxy or protected hydroxy; R₄ is hydrogen or C₁₋₉ alkyl; and salts thereof; have useful pharmacological activity.

A novel class of compounds also having useful pharmacological activity has now been discovered, which compounds are structurally distinct from the prior art referred to above.

Accordingly the present invention provides a compound of the formula (I): ##STR5## wherein: X is O or S;

n is 1 to 8;

R₁ is hydrogen, or CO₂ R₁ represents an ester group in which the R₁ moiety contains from 1-12 carbon atoms;

R₂ is hydrogen, C₁₋₄ alkyl, or phenyl;

R₃ is hydroxy or protected hydroxy;

R₄ is hydrogen, C₁₋₉ alkyl, C₃₋₈ cycloalkyl, C₃₋₈ cycloalkyl-C₁₋₆ alkyl, phenyl, phenyl C₁₋₆ alkyl, naphthyl, naphthyl-C₁₋₆ -alkyl, any of which phenyl or naphthyl moieties may be substituted by one or more halogen, trifluoromethyl, C₁₋₆ alkyl, hydroxy, C₁₋₆ alkoxy, phenyl C₁₋₆ alkoxy or nitro groups; or

R₂ and R₄ taken with the carbon atom to which they are joined represent a C₅₋₈ cycloalkyl group;

R₅ is C₁₋₆ alkyl, C₁₋₆ alkyl substituted by a nitro, hydroxy, C₁₋₆ alkoxy, CO₂ A, (CO₂ A)₂, CN or halogen group, C₅₋₈ cycloalkyl, phenyl, phenyl-C₁₋₆ alkyl, phenyl-C₃₋₆ cycloalkyl, any of which phenyl moieties may be substituted by one or more halogen, trifluoromethyl, C₁₋₆ alkyl, C₁₋₆ alkoxy or nitro groups; or a group CO₂ A; in R₅ when present A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

A group of compounds within formula (I) include those wherein:

X is O or S;

n is 4 to 8;

R₁ is hydrogen, or CO₂ R₁ represents an ester group in which the R₁ moiety contains from 1 to 12 carbon atoms;

R₂ is hydrogen, C₁₋₄ alkyl, or phenyl;

R₃ is hydroxy or protected hydroxy;

R₄ is hydrogen, C₁₋₉ alkyl, C₅₋₈ cycloalkyl, C₅₋₈ cycloalkyl-C₁₋₆ alkyl, phenyl, phenyl C₁₋₆, alkyl, napthyl, napthyl-C₁₋₆ -alkyl, any of which phenyl or naphthyl moieties may be substituted by one or more halogen, trifluoromethyl, C₁₋₆ alkyl, C₁₋₆ alkoxy or nitro groups;

R₅ is C₁₋₆ alkyl, phenyl, phenyl-C₁₋₆ alkyl, or a group CO₂ A wherein A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

Particularly suitable compounds within formula (I) include those where X is O.

Suitably n is 5, 6 or 7, preferably 6.

R₁ is hydrogen or CO₂ R₁ represents an ester group in which the R₁ moiety contains from 1 to 12 carbon atoms. Examples of R₁ include hydrogen, methyl, ethyl, n- and iso-propyl, n-, sec- and tert-butyl, phenyl, benzyl, toluyl and the like, while normally hydrogen or C₁₋₆ alkyl groups are preferred.

Suitable examples of R₂ include hydrogen, methyl, ethyl and phenyl. More suitably R₂ is hydrogen, methyl or ethyl, preferably methyl.

Suitable protected hydroxyl groups R₃ include readily hydrolysable groups such as acylated hydroxy groups in which the acyl moiety contains 1 to 4 carbon atoms, for example the acetoxy group; and hydroxy groups etherified by readily removable inert groups such as the benzyl group or like groups. Preferably R₃ is hydroxyl.

Suitable groups R₄ when R₄ is an alkyl group include C₄₋₉ alkyl groups. Such C₄₋₉ alkyl groups may be straight chain alkyl groups, such n-butyl, n-pentyl, n-hexyl and n-heptyl, or may be alkyl groups branched by one or two methyl groups (at the same or different carbon atoms). Thus for example, R₄ may be a group CH₂ R₇, CH(CH₃)R₇ or C(CH₃)₂ R₇, wherein R₇ is a straight chain alkyl group such that the carbon content of the resultant group R₄ is 4 to 9.

In general preferred groups R₄ when R₄ is an alkyl group include straight chain pentyl, hexyl and heptyl groups. Of these, straight chain hexyl is often the most useful. Other preferred groups R₄ include groups CH(CH₃)R₇ and C(CH₃)₂ R₇ wherein R₇ is straight chain butyl, pentyl and hexyl.

Other suitable examples of R₄ when R₄ is an alkyl group include the lower alkyl groups, that is when R₄ is a C₁₋₄ alkyl group.

When R₄ is or contains a C₃₋₈ cycloalkyl moiety, the moiety may be cyclopropyl. The moiety may also be a C₅₋₈ cycloalkyl moiety such as a cyclohexyl moiety. Examples of suitable C₁₋₆ alkyl moieties when R₄ is a C₃₋₈ cycloalkyl-C₁₋₆ alkyl group include methyl, ethyl, propyl, butyl and amyl.

When R₄ is an aryl group as previously defined, suitable groups R₄ include phenyl, phenylmethyl, phenylethyl, phenyl n-propyl, phenyl n-butyl, naphthyl, naphthyl-methyl, naphthyl-ethyl, naphthyl n-propyl and naphthyl n-butyl, and such groups branched in the alkyl moiety by one or two methyl groups (at the same or different carbon atoms). These groups may be substituted in the phenyl or naphthyl moiety by normally one, two or three groups selected from those substituent groups listed hereinbefore. Examples of suitable substituent groups include fluorine, chlorine and bromine atoms and CF₃, methyl, ethyl, n- and iso-propyl, methoxy and ethoxy, n- and iso-propoxy and nitro groups. Other examples of such groups include hydroxy and benzyloxy. Preferably the aryl moieties when substituted by such groups will be mono or di-substituted.

Also, R₂ and R₄ taken with the carbon atom to which they are joined can represent a C₅₋₈ cycloalkyl group, such as the cyclohexyl group.

Suitable examples of R₅ include methyl, ethyl, n- and iso-propyl, n-, sec- and tert-butyl; phenyl; phenylmethyl, phenylethyl, phenyl-n-propyl, phenyl-n-butyl, and such phenylalkyl groups branched in their alkyl moities by one or two methyl groups (at the same or different carbon atoms). More suitably R₅ is C₁₋₆ alkyl such as methyl and ethyl.

R₅ may also be a phenyl-C₃₋₆ cycloalkyl group, in which case suitable examples of R₅ include phenylcyclopropyl.

When R₅ is or includes a phenyl moiety, it can optionally be substituted as described above for R₄ aryl groups.

When R₅ is C₅₋₈ cycloalkyl, it is suitably cyclohexyl.

When R₅ is, or contains, a group CO₂ A, suitable examples of A include hydrogen, methyl, ethyl, n- and iso-propyl, n-, sec- and tert-butyl, phenyl, benzyl, toluyl and the like, while normally for A hydrogen or C₁₋₆ alkyl are preferred.

R₅ may also be a C₁₋₆ alkyl group substituted by a nitro, hydroxy, C₁₋₆ alkoxy (such as methoxy), CO₂ A, (CO₂ A)₂, CN or halogen group. In such cases often R₅ will be a methylene group substituted by one of these groups.

The compounds of the formula (I) may form conventional salts. Such salts include those with alkali and alkaline earth metals, suitably sodium and potassium, and ammonium and substituted ammonium salts.

From the aforesaid it will be seen that one particularly suitable group of compounds within formula (I) is of formula (II): ##STR6## wherein: X and R₁ are as defined in formula (I);

n¹ is 5, 6 or 7;

R¹ ₂ is hydrogen, methyl, ethyl or phenyl;

R¹ ₄ is hydrogen or C₁₋₉ alkyl;

R¹ ₅ is C₁₋₆ alkyl, phenyl, phenyl-C₁₋₆ alkyl, or a group CO₂ A wherein A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

In formula (II) n¹ is preferably 6. Also suitably X is O.

R¹ ₂ is more suitably hydrogen, methyl or ethyl, preferably methyl.

While R¹ ₄ may be hydrogen or a C₁₋₉ alkyl group, it is normally a C₄₋₉ alkyl group. In such cases suitable and preferred straight chain and branched groups R¹ ₄ include those previously described as suitable and preferred for the groups R₄ when R₄ is a C₄₋₉ alkyl group. Such preferred groups R¹ ₄ include straight chain pentyl, hexyl and heptyl, and of these normally the most useful is straight chain hexyl. Other preferred groups R¹ ₄ include CH(CH₃)R¹ ₇ and C(CH₃)₂ R¹ ₇ wherein R¹ ₇ is straight chain butyl, pentyl or hexyl.

Suitably R¹ ₅ is C₁₋₆ alkyl such as methyl and ethyl.

A second group of compounds within formula (I) of particular interest are those of formula (III): ##STR7## wherein: X and R₁ are as defined in formula (I);

n¹ is 5, 6 or 7;

R¹ ₂ is hydrogen, methyl, ethyl or phenyl;

R² ₄ is a group of formula (IV): ##STR8## wherein T is a bond, or a C₁₋₆ alkylene group which may be straight chain or branched by one or two methyl groups at the same or different carbon atoms; and W, Y and Z are each hydrogen or fluorine, chlorine or bromine atoms, or CF₃, methyl, ethyl, n- or iso-propyl, methoxy, ethoxy, n- or iso-propoxy or nitro groups;

R¹ ₅ is C₁₋₆ alkyl, phenyl, phenyl-C₁₋₆ alkyl, or a group CO₂ A wherein A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

In formula (III) n¹ is preferably 6. Also suitably X is O.

R¹ ₂ is more suitably hydrogen, methyl or ethyl, preferably methyl.

In formula (IV) often T will be a group --(CH₂)_(q) -- wherein q is 0 to 4. Also suitably W and Y are hydrogen.

Suitably R¹ ₅ is C₁₋₆ alkyl such as methyl and ethyl.

Another group of compounds within formula (I) of particular interest is of formula (V): ##STR9## wherein: X and R₁ are as defined in formula (I);

n¹ is 5, 6 or 7;

R¹ ₂ is hydrogen, methyl, ethyl or phenyl;

R³ ₄ is a group of formula (VI): ##STR10## wherein T, W, Y and Z are as defined in formula (IV);

R¹ ₅ is C₁₋₆ alkyl, phenyl, phenyl-C₁₋₆ alkyl, or a group CO₂ A wherein A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

In formula (V) n¹ is preferably 6. Also suitably X is O.

R¹ ₂ is more suitably hydrogen, methyl or ethyl, preferably methyl.

In formula (V) often T will be a group --(CH₂)_(q) -- wherein q is 0 to 4. Also suitably W and Y are hydrogen.

Suitably R¹ ₅ is C₁₋₆ alkyl such as methyl and ethyl.

A further group of compounds within formula (I) of interest are of formula (VII): ##STR11## wherein: X and R₁ are as defined in formula (I);

n¹ is 5, 6 or 7;

R¹ ₂ is hydrogen, methyl, ethyl or phenyl;

R⁴ ₄ is a group of formula (VIII): ##STR12## wherein T is as defined in formula (IV) and

r is 0 to 3;

R¹ ₅ is C₁₋₆ alkyl, phenyl, phenyl-C₁₋₆ alkyl, or a group CO₂ A wherein A is hydrogen or CO₂ A represents an ester group in which the A moiety contains from 1 to 12 carbon atoms; and salts thereof.

In formula (VII) n¹ is preferably 6. Also suitably X is O.

R¹ ₂ is more suitably hydrogen, methyl, or ethyl, preferably methyl.

In formula (VIII) often T will be a group --(CH₂)_(q) -- wherein q is 0 to 4. Also suitably r is 1.

Suitably R¹ ₅ is C₁₋₆ alkyl such as methyl and ethyl.

One compound of the invention that is particularly preferred for its useful activity is 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-methyl-5-(6"-carboxy-n-hexyl)hydantoin.

The present invention further provides a process for the preparation of the compounds of the formula (I), which process comprises the cyclisation of a compound of formula (IX): ##STR13## wherein the variable groups are as defined; and thereafter if desired or necessary converting R₁ and/or R₃ in the thus formed compound into other variables R₁ and R₃.

Compounds of the formula (IX) are conveniently prepared in situ during the reaction of a compound of the formula: ##STR14## wherein n, R₁, R₂, R₃ and R₄ are as defined, with R₅ NCX, a preferred process of the invention. This process is suitably carried out under reflux in an inert solvent such as benzene and the like. It should be stated that when in this reaction R₅ is a sterically hindered group then this reaction may proceed only as far as the uncyclised compound of formula (IX), in which case the necessary cyclisation of the compound (IX) can be achieved with a strong base, such as sodium hydride or sodium ethoxide, in a dry organic solvent. Sodium ethoxide in benzene, or potassium t-butoxide in toluene, benzene or hexamethyl phosphoramide are suitable reagents.

The conversion of a compound of the formula (I) to another compound of the formula (I) wherein R₁ and/or R₃ are altered when desired or necessary, may be achieved in conventional manner.

For example, if desired the group R₁ in the compound may be varied by conventional esterification and/or de-esterification reactions. Similarly protected R₃ hydroxy moieties may be deprotected in conventional manner. For example when R₃ is a benzyloxy group, the benzyl group may readily be removed by hydrogenolysis. Thus it may be seen that `protected hydroxy` compounds of the formula (I) are useful intermediates in the preparation of the corresponding `free hydroxy` compounds of the formula (I). Also when a compound of the formula (I) contains an acidic hydrogen atom, salts thereof may be prepared in conventional manner for example by reacting the compound of the formula (I) with the required base.

Also intermediates of the formula (I) which however are unsubstituted in the 3-position may be converted to the corresponding compounds of this invention wherein R₅ is as defined above by conventional substitution reactions with R₅ X wherein X is a displaceable group such as a halide or other good leaving group. In such reactions it may be necessary to first convert the unsubstituted compound to an alkali metal salt.

The skilled man will realise that in some such cases substitutions will also substitute a R₁ hydrogen. Thus if a compound is desired wherein R₁ is hydrogen, in such cases it will be preferred to esterify the R₁ hydrogen before the substitution reaction, and then de-esterify after the substitution reaction, to give the desired R₁ hydrogen compound.

Intermediates of formula (I) which are unsubstituted in the 3-position can be obtained through the reaction of a compound of formula (X) with an isocyanate or isothiocyanate.

When R₁ is hydrogen, this cyclisation may suitably be carried out by reacting a salt M⁺ C⁻ NX, wherein M⁺ is a metal ion and X is O or S as defined, with a compound of formula (X). The metal salt of a compound of formula (IX) thus obtained can be converted to the acid of formula (IX) with mineral acid, and then the cyclisation completed in aqueous conditions at acid pH, for example in 25% aqueous acid. Suitably M⁺ is a sodium or potassium ion, preferably a potassium ion.

When R₁ is other than hydrogen a compound of formula (X) is cyclised by reaction with M⁺ C⁻ NX. This conversion may suitably be achieved using a hydrochloride salt of the compound of the formula (X) and reacting that salt in aqueous solution at reflux, or in aqueous dichloromethane with a phase transfer catalyst.

It is believed that the compounds of formula (IX) are novel, and thus they form an important part of this invention as intermediates.

The compounds of the formula (X) may be prepared by the method disclosed in Belgian Pat. No. 835989, or by analogous methods thereto.

It will of course be realised that the compounds of the formula (I) have asymmetric centres, and thus are capable of existing in a number of stereoisomeric forms. The invention extends to each of these stereoisomeric forms, and to mixtures thereof. The different stereoisomeric forms may be separated one from the other by the usual methods.

Compounds within the formula (I) have useful pharmacological activity. For example compounds within the formula (I) have anti-gastric secretion activity e.g. anti-ulcer activity, cardiovascular activity e.g. anti-hypertensive activity, platelet aggregration inhibition activity, affect the respiratory tract e.g. bronchodilator activity, and have anti-fertility, smooth muscle and anti-arrythmic activity.

In general it may be said that compounds within the formula (I) have a range of pharmacological activities similar to those shown by the natural prostaglandins, but that these activities tend to be rather more selective.

The invention therefore also provides a pharmaceutical composition comprising a compound of the formula (I) and a pharmaceutically acceptable carrier.

Clearly the formulation of the said pharmaceutical composition will depend on the nature of the activity shown by the chosen compound of the formula (I), and on other factors such as a preference in a particular area of therapy for a particular mode of administration.

The compositions may be in the form of tablets, capsules, powders, granules, lozenges or liquid preparations, such as oral or sterile parenteral solutions of suspensions.

Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, fillers, tabletting lubricants, disintegrants, and acceptable wetting agents and the like. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), preservatives, and if desired conventional flavouring or colouring agents, and the like.

For parenteral administration, fluid unit dosage forms are prepared utilizing the compound of the formula (I) and a sterile vehicle. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved for injection and filter sterilized before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents can be dissolved in the vehicle. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The compound can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound.

When appropriate, the compositions of this invention may be presented as an aerosol for oral administration, or as a microfine powder for insufflation.

As is common practice, the compositions will usually be accompanied by written or printed directions for use in the medical treatment concerned.

It will of course be realised that the precise dosage used in the treatment of any of the hereinbefore described disorders will depend on the actual compound of the formula (I) used, and also on other factors such as the seriousness of the disorder being treated.

The invention also provides a method of treatment and/or propylaxis of disorders in human beings or animals which comprises the administration to the sufferer of an effective amount of a compound of the formula (I).

Normally however the compounds will be used in the therapy of human disorders.

The following Examples illustrate the preparation of compounds of the formula (I) and their pharmacological properties.

EXAMPLE 1 ##STR15##

Dimethyl 2-[N-3'-hydroxy-3'-methyl-n-decyl]aminoazelate (9.6 g) was refluxed with methyl iso-cyanate (1.365 g) for 3 hours in dry benzene (80 mls). The benzene was evaporated in vacuo to give a pale yellow gum (10.2 g). This was chromatographed on kieselgel (packing ratio 30:1), using chloroform as eluant to give 1-(3'-hydroxy-3'-methyl-n-decyl)-3-methyl-5-(6"-methoxycarbonyl-n-hexyl)hydantoin as a clear oil (6 g).

The compounds shown in Table 1 were prepared in a similar manner.

                  Table I                                                          ______________________________________                                          ##STR16##                                                                     Compound                                                                       number  R.sub.1                                                                               R.sub.2                                                                               R.sub.4      R.sub.5 n                                   ______________________________________                                         2       C.sub.2 H.sub.5                                                                       CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            C.sub.2 H.sub.5                                                                        6                                   3       C.sub.2 H.sub.5                                                                       CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            CH.sub.3                                                                               6                                   4       CH.sub.3                                                                               ##STR17##        CH.sub.3                                                                               6                                     5       CH.sub.3                                                                              CH.sub.3                                                                              (CH.sub.2).sub.2 Ph                                                                         CH.sub.3                                                                               6                                   6       CH.sub.3                                                                              CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            CH.sub.3                                                                               6                                   7       CH.sub.3                                                                              CH.sub.3                                                                              CH(CH.sub.3)C.sub.4 H.sub.9                                                                 CH.sub.3                                                                               6                                   8       CH.sub.3                                                                              CH.sub.3                                                                              Ph           CH.sub.3                                                                               6                                   9       CH.sub.3                                                                              CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            Ph      6                                   10      CH.sub.3                                                                              CH.sub.3                                                                               ##STR18##   CH.sub.3                                                                               6                                   11      CH.sub.3                                                                              CH.sub.3                                                                              CH.sub.3     CH.sub.3                                                                               6                                   12      CH.sub.3                                                                              CH.sub.3                                                                              C.sub.6 H.sub.13                                                                             ##STR19##                                                                             6                                   35      CH.sub.3                                                                              CH.sub.3                                                                              C.sub.6 H.sub.13                                                                             ##STR20##                                                                             6                                   36      C.sub.2 H.sub.5                                                                       CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            CH.sub.3                                                                               7                                   37      C.sub.2 H.sub.5                                                                       CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            CH.sub.3                                                                               5                                   38      CH.sub.3                                                                              CH.sub.3                                                                              C.sub.6 H.sub.13                                                                            CH.sub.3                                                                               1                                   ______________________________________                                    

example 2 ##STR21##

Dimethyl 2-[N-(3'-hydroxy-3'-methyl)-n-nonyl]aminoazelate (20 g) was refluxed with t-butyl isocyanate (5.12 g) in dry benzene (200 ml) for 3 hours. The benzene was evaporated in vacuo to give a pale yellow gum (20 g). The gum was chromatographed on kieselgel (600 g) using chlorogorm as eluant to give dimethyl 2-[N-(3'-hydroxy-3'-methyl-n-nonyl)-N-(N'-t-butylformamido)]aminoazelate (11.6 g) as a clear gum.

EXAMPLE 3 ##STR22##

Dimethyl 2-[N-(3'-hydroxy-3'-methyl-n-nonyl)-N-(N'-t-butylformamido)]aminoazelate (10 g) was refluxed with potassium t-butoxide (2.5 g) in dry toluene (150 ml) for 3 hours. The toluene was evaporated in vacuo and the resulting gum was partitioned between ether and very dilute hydrochloric acid. The ether solution was washed with brine then dried (MgSO₄) and evaporated in vacuo to give a yellow gum (8.4 g). The gum was chromatographed on kieselgel (250 g) using chloroform as eluant to give 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-t-butyl-5-(6"-methoxycarbonyl-n-hexyl)hydantoin (5.2) as a clear gum.

EXAMPLE 4 ##STR23##

Dimethyl 2-[N-3'-benzyloxy-n-nonyl]aminoazelate (20 g) was refluxed with methyl iso-cyanate (2.46 g) in dry benzene (200 ml) for 3 hours. The benzene was evaporated in vacuo to give a deep yellow oil which was chromatographed on kieselgel (30:1 packing ratio), using chloroform as eluant, to give 1-(3'-benzyloxy-n-nonyl)3-methyl-5-(6"-methoxycarbonyl-n-hexyl)hydantoin (9 g) as a colourless gum.

EXAMPLE 5 ##STR24##

1-(3'-hydroxy-3'-methyl-n-nonyl)-3-ethyl-5-(6"-ethoxycarbonyl-n-hexyl)hydantoin (1.1 g) was refluxed overnight with 10% aqueous potassium carbonate solution (7.5 ml) and ethanol (30 ml). The solution was cooled and acidified with concentrated hydrochloric acid. The product was extracted into ether (3 × 100 ml). The ether solution was extracted with 5% sodium bicarbonate solution. The resulting aqueous phase was back-washed with ether then was acidified with dilute hydrochloric acid. The product was extracted into ether and the ether solution was washed with brine, dried (MgSO₄) and evaporated in vacuo to give 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-ethyl-5-(6"-carboxy-n-hexyl)hydantoin as a colourless gum (770 mg).

The compounds shown in Table 2 were prepared in a similar manner.

                  Table 2                                                          ______________________________________                                          ##STR25##                                                                     Compound                                                                       number  R.sub.2                                                                               R.sub.4           R.sub.5 n                                     ______________________________________                                         17      CH.sub.3                                                                              C.sub.7 H.sub.15  CH.sub.3                                                                               6                                     18      CH.sub.3                                                                              C.sub.2 H.sub.5   CH.sub.3                                                                               6                                     19                                                                                      ##STR26##           CH.sub.3  6                                       20      CH.sub.3                                                                              (CH.sub.2).sub.2 Ph                                                                              CH.sub.3                                                                               6                                     21      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.3                                                                               6                                     22      CH.sub.3                                                                              CH(CH.sub.3)C.sub.4 H.sub.9                                                                      CH.sub.3                                                                               6                                     23      CH.sub.3                                                                              Ph                CH.sub.3                                                                               6                                     24      H      C.sub.6 H.sub.13  CH.sub.3                                                                               6                                     25      CH.sub.3                                                                              C.sub.6 H.sub.13  C(CH.sub.3).sub.3                                                                      6                                     26      CH.sub.3                                                                              C.sub.6 H.sub.13  Ph      6                                     27      CH.sub.3                                                                               ##STR27##        CH.sub.3                                                                               6                                     28      CH.sub.3                                                                              CH.sub.3          CH.sub.3                                                                               6                                     29      CH.sub.3                                                                              C.sub.6 H.sub.13                                                                                  ##STR28##                                                                             6                                     39      CH.sub.3                                                                              C.sub.6 H.sub.13                                                                                  ##STR29##                                                                             6                                     40      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.3                                                                               7                                     41      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.3                                                                               5                                     42      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.3                                                                               1                                     48      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.2 OCH.sub.3                                                                     6                                     49      CH.sub.3                                                                              C.sub.6 H.sub.13  CH.sub.2 CO.sub.2 H                                                                    6                                     ______________________________________                                    

example 6 ##STR30##

Dimethyl 2-[N-3'-hydroxy-3'-methyl-n-nonyl]aminoazelate (10 g) was refluxed with methyl iso-thiocyanate (1.89 g) in dry toluene (100 ml) for 3 hours. The toluene was evaporated in vacuo to give a yellow oil (11.1 g). The oil was chromatographed on kieselgel (330 g) using chloroform as eluant to give 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-methyl-5-(6"-methoxycarbonyl-n-hexyl)-2-thiohydantoin (9.49 g) as a pale yellow oil.

The compounds shown in Table 3 were prepared in a similar manner:

                  Table 3                                                          ______________________________________                                          ##STR31##                                                                     Compound No.                                                                               R.sub.1  R.sub.2  R.sub. 4                                                                               R.sub.5                                  ______________________________________                                         31          C.sub.2 H.sub.5                                                                         CH.sub.3 C.sub.6 H.sub.13                                                                       CH.sub.3                                 32          CH.sub.3 CH.sub.3 CH.sub.3                                                                               CH.sub.3                                 ______________________________________                                    

example 8 ##STR32##

1-(3'-Benzyloxy-n-nonyl)-3-methyl-5-(6"-methoxycarbonyl-n-hexyl)hydantoin (5g) was hydrogenolysed over 10% palladium/charcoal, in dry dimethoxyethane (50 mls), at room temperature and atmospheric pressure. The reaction mixture was then filtered through kieselguhr and the dimethoxyethane was evaporated in vacuo to give a colourless oil (3.8g). The oil was chromatographed on silica gel (110g) using chloroform as eluant to give 1-(3'-hydroxy-n-nonyl)-3-methyl-5-(6"-methoxycarbonyl-n-hexyl)hydantoin as a colourless oil (2.48g).

EXAMPLE 9 ##STR33##

Dry hydrogen chloride gas was passed into an ice-cold solution of dimethyl 2-[N-(3'-hydroxy-3'-methyl)-n-nonyl] aminoazelate (40 g) in dry ether (1 l). The ether was evaporated in vacuo and the resulting hydrochloride was stirred with water (300 ml). A solution of potassium cyanate (8.2 g; 1.01 eq) in water (20 ml) was added and the resulting suspension was stirred at room temperature for 1.5 hours then at reflux for 1.5 hours. The mixture was allowed to cool and the product was extracted into dichloromethane. The dichloromethane solution was washed with brine until the washings were neutral then was dried and evaporated to give a yellow gum (38 g). A sample was purified via column chromatography (silica gel; 30:1) using chloroform, and chloroform methanol mixtures as eluants to give 1-(3'-hydroxy-3'-methyl-n-nonyl)-5-(6"-methoxycarbonyl-n-hexyl) hydantoin as a pale yellow gum.

EXAMPLE 9 ##STR34##

1-(3'-Hydroxy-3'-methyl-n-nonyl)-5-(6"-methoxycarbonyl-n-hexyl) hydantoin (5g) in dry dimethylformamide (10 ml) was added to a stirred suspension of sodium hydride (376 mg; 80% oil dispersion) in dry dimethyl-formamide (20 ml), under nitrogen, at room temperature. The mixture was stirred overnight. Chloromethyl methyl ether (1.01 g) in dry dimethylformamide (10 ml) was added dropwise and the mixture was stirred for 24 hours at room temperature. The product was partitioned between very dilute hydrochloric acid and ether. The ether solution was washed with 5% aqueous sodium hydroxide solution, and with brine until the washings were neutral, then was dried and evaporated in vacuo to give a yellow oil (4.1 g). The oil was chromatographed on silica gel (30:1) using chloroform, 1% methanol/chloroform and 2% methanol/chloroform as eluants to give 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-methoxymethyl-5-(6"-methoxycarbonyl-n-hexyl) hydantoin (2.5 g) as a pale yellow gum.

The compounds prepared in Table 7 were prepared in a similar manner.

                  Table 7                                                          ______________________________________                                          ##STR35##                                                                     Compound                                                                       number    R.sub.5 X       R.sub.5                                              ______________________________________                                         45        BrCH.sub.2 CO.sub.2 CH.sub.3                                                                   CH.sub.2 CO.sub.2 CH.sub.3                           46        BrCH.sub.2 CN   CH.sub.2 CN                                           47*      BrCH(CO.sub.2 CH.sub.3).sub.2                                                                  CH(CO.sub.2 CH.sub.3).sub.2                          51        BrCH.sub.2 Ph   CH.sub.2 Ph                                          ______________________________________                                          *The alkali extraction procedure was eliminated.                         

EXAMPLE 10 ##STR36##

A 1% solution of sodium hydroxide in dry methanol (1 equivalent) was added to a solution of 1-(3'-hydroxy-3'-methyl-n-nonyl)-3-methyl-5-(6"-carboxy-n-hexyl) hydantoin in dry methanol at room temperature. The methanol was evaporated in vacuo at 30° C. and the product was triturated with 40/60 petroleum ether. The product was collected and dried over sodium hydroxide in a vacuum desiccator then was ground to a fine, pale yellow powder.

In similar manner was prepared the corresponding lithium salt.

    ______________________________________                                         Analytical Data                                                                Compound 1                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR37##                                                                     1730, [CO.sub.2 CH.sub.3 ],                                     NMR, (τ)                                                                            :     7.15, (s), [OH];                                                                ##STR38##                                                                      ##STR39##                                                                     6.35, (s). [CO.sub.2 CH.sub.3 ];                                                ##STR40##                                                      ______________________________________                                    

analysis: C₂₃ H₄₂ N₂ O₅

requires: C, 64.76; H, 9.92; N, 6.57%.

found: C, 64.44; H, 9.92; N, 6.71%.

mass Spec: C₂₃ H₄₂ N₂ O₅

requires: 426.3093

found: 426.3065

    ______________________________________                                         Compound 2                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR41##                                                                     1730, [CO.sub.2 Et]                                             NMR (τ)                                                                             :     7.2, (s) [OH]; 6.2 to 6.8, (m),                                 (CCl.sub.4)                                                                                    ##STR42##                                                                      ##STR43##                                                      ______________________________________                                    

    ______________________________________                                         compound 3                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR44##                                                                     1730, [CO.sub.2 Et]                                             NMR (τ)                                                                             :                                                                                     ##STR45##                                                                      ##STR46##                                                                      ##STR47##                                                      ______________________________________                                    

    ______________________________________                                         compound 4                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :     3500, [OH]; 1700, 1760,                                                         ##STR48##                                                                     1720, [CO.sub.2CH.sub.3 ].                                      NMR (τ)                                                                             :                                                                                     ##STR49##                                                                      ##STR50##                                                                     6.35, (s), [CO.sub.2 CH.sub.3 ]                                                 ##STR51##                                                      ______________________________________                                    

analysis: C₂₀ H₃₄ N₂ O₅

requires: C, 62.80; H, 8.96; N, 7.32%

found: C, 62.61; H, 8.95; N, 7.19%

mass Spec: C₂₀ H₃₄ N₂ O₅

requires: 382.2468

found: 382.2466

    ______________________________________                                         Compound 5                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :     3500, [OH]; 1700, 1760,                                                         ##STR52##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.75, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                      7.3, (m), [CH.sub.2 Ph];                                                       7.1, (s), [OH];                                                                 ##STR53##                                                                      ##STR54##                                                                     6.5, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR55##                                                      ______________________________________                                    

mass Spec: C₂₄ H₃₄ N₂ O₄ [m*-H₂ O]

requires: 414.2518

found: 414.2523

    ______________________________________                                         Compound 6                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR56##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.3, (s), [OH];                                                                 ##STR57##                                                                      ##STR58##                                                                     6.35, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR59##                                                      ______________________________________                                    

analysis: C₂₂ H₄₀ N₂ O₅

requires: C, 64.05; H, 9.77; N, 6.79%

found: C, 64.14; H, 9.68; N, 6.62%

mass Spec: C₂₂ H₃₈ N₂ O₄ [m*-H₂ O]

requires: 394.2832

found: 394.2848

    ______________________________________                                         Compound 7                                                                     ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR60##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.75, (m), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                      7.2, (s), [OH];                                                                 ##STR61##                                                                      ##STR62##                                                                     6.35, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR63##                                                      ______________________________________                                    

mass Spec: C₂₂ H₃₈ N₂ O₄ [m*-H₂ O]

requires: 394.2831

found: 394.2794

    ______________________________________                                         Compound 8                                                                     I.R. (cm.sup.-1)                                                                        :     3500, [OH]; 1700, 1760,                                                         ##STR64##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     Compound 9                                                                     I.R. (cm.sup.-1)                                                                        :     3500 cm.sup.-1, [OH]; 1710, 1770,                                               ##STR65##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.75, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                      7.45, (s), [OH];                                                                ##STR66##                                                                     6.37, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR67##                                                                     2.65, (s), [C.sub.6 H.sub.5 ].                                  ______________________________________                                    

mass Spec: C₂₇ H₄₂ N₂ O₅

requires: 474.3094

found: 474.3083

    ______________________________________                                          Compound 10                                                                   ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR68##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.8, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       7.3, (m), [CH.sub.2Ar];                                                         ##STR69##                                                                      ##STR70##                                                                     6.45, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR71##                                                                     2.65, (broad s), [Ar].                                          ______________________________________                                    

Mass Spec: C₂₅ H₃₃ N₂ O₄ F₃ [m*-H₂ O]

requires: 482.2392

found: 482.2415

    ______________________________________                                          Compound 11                                                                   ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                     ##STR72##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                     NMR (τ)                                                                             :     7.75, (t), [CH.sub.2CO.sub.2 CH.sub.3 ];                                       7.15, (s), [OH];                                                                ##STR73##                                                                      ##STR74##                                                                     6.4, (s), [CO.sub.2 CH.sub.3 ];                                                6.05, (m), [NCH].                                               ______________________________________                                    

mass Spec: C₁₇ H₃₀ N₂ O₅

requires: 342.2154

found: 342.2144

Mass Spec: C₁₇ H₂₈ N₂ O₄ [m*-H₂ O]

requires: 324.2049

found: 324.2050

    ______________________________________                                         Compound 12                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       ##STR75##                                                                     1740, [CO.sub.2 CH.sub.3 ].                                            NMR (τ):                                                                           7.75, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                      7.1, (s), [OH];                                                                 ##STR76##                                                                     6.4, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR77##                                                             ______________________________________                                    

mass Spec: C₂₇ H₄₈ N₂ O₅

requires: 480.3564

found: 480.3536

    ______________________________________                                         Compound 13                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3400, [OH]; 1740, [CO.sub.2 CH.sub.3 ];                                         ##STR78##                                                            ______________________________________                                    

analysis: C₂₆ H₅₀ N₂ O₆

requires: C, 64.16; H, 10.36; N, 5.76%

found: C, 64.22; H, 10.69; N, 5.43%

mass Spec: C₂₅ H₄₄ N₂ O₄ [m*-H₂ O-CH₃ OH]

requires: 436.3301

found: 436.3293

    ______________________________________                                         Compound 14                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3500, [OH];                                                                     ##STR79##                                                                     1735, [CO.sub.2 CH.sub.3 ].                                           NMR (τ):                                                                            7.2, (s), [OH];                                                                 ##STR80##                                                                     6.3, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR81##                                                            ______________________________________                                    

analysis: C₂₅ H₄₆ N₂ O₅

requires: C, 66.05; H, 10.20; N, 6.16%

found: C, 65.89; H, 10.30; N, 6.13%

mass Spec: C₂₅ H₄₆ N₂ O₅

requires: 454.3406

found: 454.3451

Mass Spec: C₂₅ H₄₄ N₂ O₄ [m*-H₂ O]

requires: 436.3301

found: 436.3317

    ______________________________________                                         Compound 15                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                        ##STR82##                                                                     1730, [CO.sub.2 CH.sub.3 ].                                           NMR (τ):                                                                             ##STR83##                                                                      ##STR84##                                                                     6.35, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR85##                                                                     5.5, (s, with shoulder), [OCH.sub.2 Ph];                                       2.75, (s), [OCH.sub.2 Ph].                                            ______________________________________                                    

Analysis: C₂₈ H₄₄ N₂ O₅

requires: C, 68.82; H, 9.08; N, 5.73%

found: C, 68.62; H, 9.21; N, 5.66%

mass Spec: C₂₈ H₄₄ N₂ O₅

requires: 488.3250

found: 488.3287

    ______________________________________                                         Compound 16                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3700, to 2500, [CO.sub.2 H; OH];                                                ##STR86##                                                            NMR (τ):                                                                             ##STR87##                                                                     4.4 (broad s), [CO.sub.2 H; OH].                                      ______________________________________                                    

mass Spec: C₂₂ H₄₀ N₂ O₅

requires: 412.2937

found: 412.2917

    ______________________________________                                         Compound 17                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3700 to 2400,                                                                   ##STR88##                                                                      ##STR89##                                                            NMR (τ):                                                                             ##STR90##                                                                      ##STR91##                                                                      ##STR92##                                                                     3.15, (broad s), [CO.sub.2 H; OH].                                    ______________________________________                                    

analysis: C₂₂ H₄₀ N₂ O₅

requires: C, 64.05; H, 9.77; N, 6.79%

found: C, 64.36; H, 9.99; N, 6.99%

mass Spec: C₂₂ H₃₈ N₂ O₄ [m*-H₂ O]

requires: 394.2831

found: 394.2848

    ______________________________________                                         Compound 18                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3700 to 2500, [CO.sub.2 H; OH];                                                 ##STR93##                                                            NMR (τ):                                                                             ##STR94##                                                            (CD.sub.3).sub.2 CO                                                                      ##STR95##                                                                      ##STR96##                                                            ______________________________________                                    

mass Spec: C₁₇ H₃₀ N₂ O₅

requires: 342.2155

found: 342.2174

    ______________________________________                                         Compound 19                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3700 to 2400, [CO.sub.2 H; OH]                                                  ##STR97##                                                            NMR (τ):                                                                             ##STR98##                                                                      ##STR99##                                                                     6.05, (broad s), [CO.sub.2 H; OH];                                              ##STR100##                                                           ______________________________________                                    

analysis: C₁₉ H₃₂ N₂ O₅

requires: C, 61.93; H, 8.75; N, 7.60%

found: C, 61.99; H, 8.97; N, 7.64%

mass Spec: C₁₉ H₃₂ N₂ O₅

requires: 368.2311

found: 368.2313

    ______________________________________                                         Compound 20                                                                    ______________________________________                                         I.R. (cm.sup.-1):                                                                       3700 to 2400, [CO.sub.2 H; OH];                                                 ##STR101##                                                           NMR (τ):                                                                            7.3, (m), [CH.sub.2 Ph];                                                        ##STR102##                                                                     ##STR103##                                                                    6.15, (s), [CO.sub.2 H; OH];                                                    ##STR104##                                                           ______________________________________                                    

analysis: C₂₃ H₃₄ N₂ O₅

requires: C, 66.01; H, 8.19; N, 6.69%

found: C, 65.82; H, 8.38; N, 6.37%

mass Spec: C₂₃ H₃₂ N₂ O₄ [m*-H₂ O]

requires: 400.2362

found: 400.2323

    ______________________________________                                         Compound 21                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3700 to 2400, [CO.sub.2 H; OH];                                                 ##STR105##                                                           NMR (τ) :                                                                            ##STR106##                                                                     ##STR107##                                                                    6.1, (s), [CO.sub.2 H; OH];                                                     ##STR108##                                                           ______________________________________                                    

mass Spec: C₂₁ H₃₆ N₂ O₄ [m*-H₂ O]

requires: 380.2675

found: 380.2672

    ______________________________________                                         Compound 22                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3700 to 2500, [CO.sub.2 H; OH];                                                 ##STR109##                                                           NMR (τ) :                                                                            ##STR110##                                                           (CD.sub.3).sub.2 CO                                                                      ##STR111##                                                                     ##STR112##                                                                    5 to 3, (broad hump), [CO.sub.2 H; OH].                               ______________________________________                                    

analysis: C₂₁ H₃₈ N₂ O₅

requires: C, 63.29; H, 9.61; N, 7.03%

found: C, 62.94; H, 9.79; N, 6.65%

mass Spec: C₂₁ H₃₆ N₂ O₄ [m*-H₂ O]

requires: 380.2675

found: 380.2641

    ______________________________________                                         Compound 23                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3700 to 2500, [CO.sub.2 H, OH];                                                 ##STR113##                                                           NMR (τ) :                                                                            ##STR114##                                                                     ##STR115##                                                                    5.6, (broad s), [CO.sub.2 H; OH];                                              2.65, (m), [Ph].                                                      ______________________________________                                    

Analysis: C₂₁ H₃₀ N₂ O₅

requires: C, 64.60; H, 7.74; N, 7.17%

found: C, 64.83; H, 7.96; N, 6.91%

mass Spec: C₂₁ H₂₈ N₂ O₄ [m*-H₂ O]

requires: 372.2049

found: 372.2037

    ______________________________________                                         Compound 24                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3700 to 2500, [CO.sub.2 H; OH];                                                 ##STR116##                                                           NMR (τ) :                                                                            ##STR117##                                                                     ##STR118##                                                                     ##STR119##                                                                    4.5, (broad), [CO.sub.2 H; OH].                                       ______________________________________                                    

analysis: C₂₀ H₃₆ N₂ O₅

requires: C, 62.47; H, 9.44; N, 7.29%

found: C, 62.40; H, 9.59; N, 7.04%

mass Spec: C₂₀ H₃₆ N₂ O₅

requires: 384.2624

found: 384.2640

    ______________________________________                                         Compound 25                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3700 to 2400, [CO.sub.2 H; OH];                                                 ##STR120##                                                           NMR (τ) :                                                                           6.7, (s), [CO.sub.2 H; OH];                                                     ##STR121##                                                                     ##STR122##                                                           ______________________________________                                    

analysis: C₂₄ H₄₄ N₂ O₅

requires: C, 65.42; H, 10.07; N, 6.36%

found: C, 65.21; H, 10.29; N, 6.08%

mass Spec: C₂₄ H₄₄ N₂ O₅

requires: 440.3250

found: 440.3280

    ______________________________________                                         Compound 25                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3200 to 2600, [CO.sub.2 H; OH];                                                 ##STR123##                                                           NMR (τ) :                                                                            ##STR124##                                                                    5.95, (broad), [CO.sub.2 H; OH];                                                ##STR125##                                                                    2.6, (d), [Ph].                                                       ______________________________________                                    

Mass Spec: C₂₆ H₃₈ N₂ O₄ [m*-H₂ O]

requires: 442.2832

found: 442.2841

    ______________________________________                                         Compound 27                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3200 to 2400, [CO.sub.2 H; OH];                                                 ##STR126##                                                           NMR (τ) :                                                                           7.8, (t), [CH.sub.2CO.sub.2 H];                                       D.sub.6 DMSO                                                                            7.4, (m), [CH.sub.2Ar];                                                         ##STR127##                                                                     ##STR128##                                                                     ##STR129##                                                           ______________________________________                                    

analysis: C₂₄ H₃₃ N₂ O₅ F₃

requires: C, 59.25; H, 6.83; N, 5.76%

found: C, 59.29; H, 7.13; N, 5.82%

mass Spec: C₂₄ H₃₁ N₂ O₄ F₃ [m*-H₂ O]

requires: 468.2236

found: 468.2245

    ______________________________________                                         Compound 28                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3200 to 2400, [CO.sub.2 H; OH];                                                 ##STR130##                                                           NMR (τ) :                                                                           7.75, (t), [CH.sub.2CO.sub.2 H];                                      (D.sub.6 DMSO)                                                                           ##STR131##                                                                     ##STR132##                                                                     ##STR133##                                                           ______________________________________                                    

analysis: C₁₆ H₂₈ N₂ O₅

requires: C, 58.52; H, 8.59; N, 8.53%

found: C, 58.59; H, 8.71; N, 8.70%

mass Spec: C₁₆ H₂₈ N₂ O₅

requires: 328.1998

found: 328.1995

    ______________________________________                                         Compound 29                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3200 to 2400, [CO.sub.2 H; OH];                                                 ##STR134##                                                           NMR (τ) :                                                                            ##STR135##                                                           (CD.sub.3).sub.2 CO                                                                     6.1, (broad s), [CO.sub.2 H; OH];                                               ##STR136##                                                                     ##STR137##                                                           ______________________________________                                    

analysis: C₂₆ H₄₆ N₂ O₅

requires: C, 66.92; H, 9.94; N, 6.00%

found: C, 66.57; H, 10.06; N, 6.25%

mass Spec: C₂₆ H₄₆ N₂ O₅

requires: 466.3406

found: 466.3403

    ______________________________________                                         Compound 30                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3510, [OH]; 1750 to 1720,                                                       ##STR138##                                                           NMR (τ) :                                                                           7.75, (m), [OH; CH.sub.2CO.sub.2 CH.sub.3 ];                                    ##STR139##                                                                     ##STR140##                                                                    6.35, (s), [CO.sub.2 CH.sub.3 ];                                               5.9, (m), [NCH].                                                      ______________________________________                                    

analysis: C₂₂ H₄₀ N₂ O₄ S

requires: C, 61.64; H, 9.41; N, 6.53; S, 7.48%

found: C, 61.71; H, 9.51; N, 6.54; S, 7.34%

mass Spec: C₂₂ H₃₈ N₂ O₃ S [m*-H₂ O]

requires: 410.2603

found: 410.2610

    ______________________________________                                         Compound 31                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                    ##STR141##                                                      NMR (τ)                                                                             :    7.7, (s), [OH];                                                                 ##STR142##                                                                     ##STR143##                                                                     ##STR144##                                                      ______________________________________                                    

analysis: C₂₃ H₄₂ N₂ O₄ S

requires: C, 62.41; H, 9.56; N, 6.33; S, 7.24%

found: C, 62.54; H, 9.85; N, 6.05; S, 7.35%

mass Spec: C₂₃ H₄₂ N₂ O₄ S

requires: 442.2865

found: 442.2866

    ______________________________________                                         Compound 32                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                           :    3500, [OH]; 1740, 1720,                                                         ##STR145##                                                   NMR (τ) :    7.45, (s), [OH];                                                                ##STR146##                                                                     ##STR147##                                                                    6.4, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR148##                                                   ______________________________________                                    

mass Spec: C₁₇ H₃₀ N₂ O₄ S

requires: 358.1926

found: 358.1956

    ______________________________________                                         Compound 34                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                           :    3500, [OH]; 1760, 1710,                                                         ##STR149##                                                   NMR (τ) :                                                                                    ##STR150##                                                                     ##STR151##                                                                    6.35, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR152##                                                   ______________________________________                                    

analysis: C₂₁ H₃₈ N₂ O₅

requires: C, 63.29; H, 9.61; N, 7.03%

found: C, 63.61; H, 9.83; N, 7.34%

mass Spec: C₂₁ H₃₈ N₂ O₅

requires: 398.2780

found: 398.2769

    ______________________________________                                         Compound 35                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                           :    3550, [OH]; 1770, 1730 (broad)                                                  ##STR153##                                                   NMR (τ) :    7.75 (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       7.35, (m), [Ph-CH];                                                            7.35, (s), [OH];                                                                ##STR154##                                                                    6.4, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR155##                                                                    2.7, (s), [Ph].                                               ______________________________________                                    

Mass Spec: C₃₀ H₄₄ N₂ O₄ (m*-H₂ O)

requires: 496.3301

found: 496.3303

    ______________________________________                                         Compound 36                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                    ##STR156##                                                                    1730, [CO.sub.2 C.sub.2 H.sub.5 ].                               NMR (τ)                                                                             :    7.7 (t), [CH.sub.2 CO.sub.2 C.sub.2 H.sub.5 ];                                 7.5, (s), [OH];                                                                 ##STR157##                                                                     ##STR158##                                                                     ##STR159##                                                                     ##STR160##                                                      ______________________________________                                    

mass Spec: C₂₄ H₄₂ N₂ O₄ (m*-H₂ O)

requires: 422.3144

found: 422.3156

    ______________________________________                                         Compound 37                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                    ##STR161##                                                                    1720, [CO.sub.2 C.sub.2 H.sub.5 ].                               NMR (τ)                                                                             :    7.75, (t), [CH.sub.2 CO.sub.2 C.sub.2 H.sub.5 ];                               7.5, (s), [OH];                                                                 ##STR162##                                                                     ##STR163##                                                                     ##STR164##                                                                    5.9, (q), [CO.sub.2 CH.sub.2 CH.sub.3 ].                         ______________________________________                                    

mass Spec: C₂₂ H₃₈ N₂ O₄ (m*-H₂ O)

requires: 394.2832

found: 394.2826

    ______________________________________                                         Compound 38                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :                                                                                    ##STR165##                                                                    1740, [CO.sub.2 CH.sub.3 ].                                      NMR (τ)                                                                             :    7.75, (s), [OH];                                                               7.1, (m), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                        ##STR166##                                                                     ##STR167##                                                                    6.25, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR168##                                                      ______________________________________                                    

mass Spec: C₁₇ H₂₈ N₂ O₄ (m*-H₂ O)

requires: 324.2048

found: 324.2056

    ______________________________________                                         Compound 39                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3700 to 2500, [CO.sub.2 H; OH];                                                 ##STR169##                                                      NMR (τ)                                                                             :    7.7, (m), [CH.sub.2 CO.sub.2 H];                                               7.3 (m), (Ph-CH];                                                               ##STR170##                                                                     ##STR171##                                                                    5.7, (broad s), [CO.sub.2 H; OH];                                              2.75, (s), [Ph].                                                 ______________________________________                                    

Mass Spec: C₂₉ H₄₂ N₂ O₄ (m*-H₂ O)

requires: 482.3145

found: 482.3184

Analysis: C₂₉ H₄₄ N₂ O₅

requires: C, 69.57; H, 8.86; N, 5.60%.

found: C, 69.83; H, 9.05; N, 5.32%.

    ______________________________________                                         Compound 40                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3800 to 2500, [CO.sub.2 H; OH];                                                 ##STR172##                                                      NMR (τ)                                                                             :    7.7, (t), [CH.sub.2 CO.sub.2 H];                                                ##STR173##                                                                     ##STR174##                                                                    6.2, (broad s), [CO.sub.2 H; OH];                                               ##STR175##                                                      ______________________________________                                    

mass Spec: C₂₂ H₃₈ N₂ O₄ (m*-H₂ O)

requires: 394.2831

found: 394.2823

Analysis: C₂₂ H₄₀ N₂ O₅

requires: C, 64.05; H, 9.77; N, 6.79%.

found: C, 63.98; H, 9.97; N, 6.57%.

    ______________________________________                                         Compound 41                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3700 to 2500, [CO.sub.2 H; OH];                                                 ##STR176##                                                      NMR (τ)                                                                             :    7.8, (t), [CH.sub.2 CO.sub.2 H];                                                ##STR177##                                                                     ##STR178##                                                                     ##STR179##                                                                    4.6 to 3.3, (hump), [CO.sub.2 H; OH].                            ______________________________________                                    

mass Spec: C₂₀ H₃₄ N₂ O₄ (m*-H₂ O)

requires: 366.2518

found: 366.2513

Compound 42

Mass Spec: C₁₆ H₂₆ N₂ O₄ (m*-H₂ O)

requires: 310.1892

found: 310.1891

    ______________________________________                                         Compound 43                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3500, [OH]; 3300, [NH];                                                         ##STR180##                                                                    1730, [CO.sub.2 CH.sub.3 ].                                      NMR (τ)                                                                             :    7.85, (m), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       ##STR181##                                                                    6.4, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR182##                                                      ______________________________________                                    

mass Spec: C₂₁ H₃₆ N₂ O₄ (m*-H₂ O)

requires: 380.2675

found: 380.2659

    ______________________________________                                         Compound 44                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3500, [OH]; 1770, 1720 (broad),                                                 ##STR183##                                                      NMR (τ)                                                                             :    7.8, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       7.3, (s), [OH];                                                                 ##STR184##                                                                    6.6, (s), (OCH.sub.3 ];                                                        6.3, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR185##                                                                    5.2, (s), [CH.sub.2 OCH.sub.3 ].                                 ______________________________________                                    

mass Spec: C₂₃ H₄₀ N₂ O₅ (m*-H₂ O)

requires: 424.2937

found: 424.2942.

    ______________________________________                                         Compound 45                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3550, [OH]; 1760 to 1710, (broad),                                              ##STR186##                                                      NMR (τ)                                                                             :    7.75, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                      7.4, (s), [OH];                                                                 ##STR187##                                                                    6.4, (s), [CO.sub.2 CH.sub.3 ];                                                6.25, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR188##                                                      ______________________________________                                    

mass Spec: C₂₄ H₄₀ N₂ O₆ (m*-H₂ O)

requires: 452.2886

found: 452.2892

    ______________________________________                                         Compound 46                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3500, [OH]; 1765, 1720 (broad),                                                 ##STR189##                                                      NMR (τ)                                                                             :    7.7, (t), [CH.sub.2 CO.sub.2 CH.sub.3 [;                                       7.3, (s), [OH];                                                                 ##STR190##                                                                    6.35, (s), [CO.sub.2 CH.sub.3 ];                                                ##STR191##                                                                    5.65, (s), [CH.sub.2 CN].                                        ______________________________________                                    

mass Spec: C₂₃ H₃₇ N₃ O₄ (m*-H₂ O)

requires: 419.2784

found: 419.2771

Analysis: C₂₃ H₃₉ N₃ O₅

requires: C, 63.13; H, 8.98; N, 9.60%.

found: C, 63.21; H, 9.13; N, 9.31%.

    ______________________________________                                         Compound 47                                                                    ______________________________________                                         I.R. (cm.sup.-1)                                                                        :    3500, [OH]; 1730, (very broad),                                                 ##STR192##                                                      NMR (τ)                                                                             :    7.75 (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       7.6, (s), [OH];                                                                 ##STR193##                                                                    6.4, (s), [CO.sub.2 CH.sub.3 ];                                                 ##STR194##                                                                     ##STR195##                                                                     ##STR196##                                                      ______________________________________                                    

mass Spec: C₂₆ H₄₂ N₂ O₈ (m*-H₂ O)

requires: 510.2941

found: 510.2937

    ______________________________________                                         Compound 48                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3200 to 2600, [CO.sub.2 H; OH];                                                 ##STR197##                                                           NMR (τ) :                                                                           7.7, (t), [CH.sub.2 CO.sub.2 H];                                                ##STR198##                                                                    6.65, (s), [OCH.sub.3 ];                                                        ##STR199##                                                                     ##STR200##                                                                    4.3, (broad s), [CO.sub.2 H; OH].                                     ______________________________________                                    

mass Spec: C₂₁ H₃₄ N₂ O₄ (m*-H₂ O-MeOH)

requires: 378.2519

found: 378.2507

    ______________________________________                                         Compound 49                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                      3600 to 2400, [CO.sub.2 H; OH];                                                 ##STR201##                                                           ______________________________________                                    

compound 50

Analysis: C₂₁ H₃₇ N₂ O₅ Na

requires: C, 59.98; H, 8.87; N, 6.66; Na, 5.47%.

found: C, 60.15; H, 9.19; N, 6.71; Na, 5.52%.

    ______________________________________                                         Compound 51                                                                    ______________________________________                                         I.R. (cm.sup.-1) :                                                                       ##STR202##                                                                    1730, [CO.sub.2 CH.sub.3 ].                                           NMR (γ) :                                                                         7.8, (t), [CH.sub.2 CO.sub.2 CH.sub.3 ];                                       7.2, (hump), [OH];                                                             7 to 6.2 approx, (m), [NCH.sub.2];                                             6.4, (s), [CO.sub.2 CH.sub.3 ];                                                6.05, (t), [NCH];                                                              5.45, (s), [CH.sub.2 Ph];                                                      2.7, (broad s), [CH.sub.2 Ph].                                        ______________________________________                                    

PHARMACOLOGICAL DATA Bronchodilation activity

1. The compounds were examined for their ability to inhibit 5-hydroxytryptamine or histamine induced bronchoconstriction in the anaesthetised, artificially respired guinea pig (Konzett-Rossler preparation). The compounds were administered intravenously. The results are shown in Table A.

2. the compounds were also examined for their ability to protect conscious guinea pigs against bronchoconstriction induced by an histamine aerosol (Herxheimer test). In these experiments the compounds were administered by aerosol or by oral administration. The results are shown in Table B. The results are the mean of several experiments.

    ______________________________________                                         Compound    ID.sub.50 against 5-hydroxytryptamine-                             number      induced constriction μg/kg, i.v.                                ______________________________________                                         1           4.5                                                                2           6.8                                                                3           0.4                                                                4           220                                                                6           1.5                                                                7           3.3                                                                16          5.8                                                                17          8.5                                                                19          >220                                                               21          0.6                                                                22          3.2                                                                23          60                                                                 24          6.0                                                                27          30                                                                 31          4.2                                                                ______________________________________                                    

Similar results were obtained against histamine-induced bronchoconstriction; for example, compound 21 also had an ID₅₀ of 0.6 μg/kg, i.v., against histamine-induced constriction.

                  TABLE B                                                          ______________________________________                                         Compound   Aerosol route  Oral route                                           number     Activity; μg/ml                                                                            Activity; mg/kg                                      ______________________________________                                         1          A ; 10         --                                                   2          A ; 10         --                                                   3          A ; 1.0        A ; 0.25                                             16         A ; 10         --                                                   17         A ; 10         --                                                   21         A ; 0.25       A ; 0.25                                             ______________________________________                                          A = active                                                               

Anti-ulcer activity Method

Anti-ulcer activity was assessed by the inhibition of indomethacin induced gastric damage in the rat according to the method of Eleghe (1974) Israeli J. Med. Sci. 10. 1451. Rats were starved overnight given 15 mg/kg indomethacin subcutaneously and sacrificed 4 hours later. Stomachs were reflated with n.saline, cut along the greater curvature pinned out and scored for gastric damage by the following system:

Score 1-3--according to degree of erythema and slight haemorrhage.

Score 4-6--according to degree of mucosal erosion.

Score 7-9--according to depth of gastric damage.

Groups of 7 rats were used for each treatment and the test compound or vehicle were administered 30 minutes prior to giving the indomethacin. Dose of test compound was 100 mg/kg orally and control groups receiving vehicle only were set up simultaneously. Mean values for each treatment were obtained using the above scoring system and the Mann Witney test applied for significance of difference between the values obtained with the treatments.

The results are shown in Table C.

                  TABLE C                                                          ______________________________________                                                  Vehicle Control Test                                                  Compound Mean Score + S.E.                                                                              Mean Score + S.E.                                     number   of Mean         of Mean                                               ______________________________________                                         17       4.86 ± 0.77  0.43 ± 0.30                                                                 (P<0.01)                                              22       2.29 ± 0.87  0.00 ± 0.00                                                                 (P<0.05)                                              25       3.86 ± 0.74  1.14 ± 0.74                                                                 (P<0.05)                                              30       2.86 ± 0.83  0.14 ± 0.14                                                                 (P<0.05)                                              31       2.00 ± 0.79  0.00 ± 0.00                                        ______________________________________                                    

Compared with vehicle only treatment, the compounds reduced the mean ulcer score and therefore have significant anti-ulcer activity.

Anti-secretory activity

The compounds were examined for their ability to inhibit pentagastrin-stimulated gastric acid secretion in the anaesthetised, perfused rat stomach preparation (Ghosh and Schild preparation). The compounds were administered intra-venously.

Compound 17 inhibited gastric acid secretion over the dose range 5-10 mg/kg, i.v.

Anti-platelet aggregation activity

The compounds were examined for their ability to inhibit platelet aggregation induced in vitro by collagen in human platelet rich plasma.

Compounds 18 and 17 inhibited aggregation by 100% and 34%, respectively, at a concentration of 100 μM. The IC₅₀ for Compound 18 against collagen-induced aggregation was 7 μM.

Anti-arrythmic activity

The compounds were examined for anti-arrythmic activity by determining their ability to prevent heart fibrillation in mice exposed to chloroform.

Compound 17, when given intraperitoneally at 100 mg/kg, prevented fibrillation induced by chloroform in a group of 3 mice.

Toxicity

The compounds do not appear to be actualy toxic. For example, Compound 17 was not toxic in mice at doses up to 300 mg/kg, orally, or at doses up to 100 mg/kg, intraperitoneally, whereas Compound 21 was not toxic at doses up to 900 mg/kg, orally, in mice. 

We claim:
 1. A compound of the formula: ##STR203## or a pharmaceutically acceptable salt thereof, wherein X is O or S;n has a value of from 1 to 8; R₁ is hydrogen; alkyl; phenyl or aralkyl of up to 12 carbon atoms; R₂ when taken alone is hydrogen; alkyl of 1 to 4 carbon atoms; or phenyl; R₃ is hydroxy, alkanoyloxy of 1 to 4 carbon atoms or benzyloxy; R₄ when taken alone is hydrogen; alkyl of 1 to 9 carbon atoms; cycloalkyl of 3 to 8 carbon atoms; phenyl; naphthyl; or alkyl of 1 to 6 carbon atoms substituted with phenyl, naphthyl or cycloalkyl of 3 to 8 carbon atoms; any of said phenyl rings and said naphthyl rings being unsubstituted or substituted with halo, trifluoromethyl, alkyl of 1 to 6 carbon atoms, hydroxy, alkoxy of 1 to 6 carbon atoms, phenylalkoxy wherein alkoxy contains from 1 to 6 carbon atoms or nitro; R₂ and R₄ taken together, together with the carbon atom to which they are joined, are cycloalkylidene of 5 to 8 carbon atoms; R₅ is alkyl of 1 to 6 carbon atoms unsubstituted or substituted with nitro, hydroxy, alkoxy of 1 to 6 carbon atoms, cyano, halo, carboxy or carbalkoxy wherein alkoxy contains up to 12 carbon atoms, cycloalkyl of 5 to 8 carbon atoms; phenyl; phenylalkyl wherein alkyl contains from 1 to 6 carbon atoms; phenylcycloalkyl wherein cycloalkyl contains 3 to 6 carbon atoms; carboxy or carbalkoxy wherein alkoxy contains up to 12 carbon atoms; any of said phenyl rings being unsubstituted or substituted with halo, trifluoromethyl, alkyl of 1 to 6 carbon atoms, alkoxy of 1 to 6 carbon atoms or nitro.
 2. A compound according to claim 1 wherein X is O.
 3. A compound according to claim 1 wherein n is 5, 6 or
 7. 4. A compound according to claim 1 wherein R₁ is hydrogen or alkyl of 1 to 6 carbon atoms.
 5. A compound according to claim 1 wherein R₂ is hydrogen or methyl.
 6. A compound according to claim 1 wherein R₃ is hydroxy.
 7. A compound according to claim 1 wherein R₄ is alkyl of 1 to 9 carbon atoms.
 8. A compound according to claim 1 wherein R₅ is alkyl of 1 to 6 carbon atoms.
 9. A compound according to claim 1 wherein R₅ is alkyl of 1 to 6 carbon atoms monosubstituted with a member of the group consisting of nitro, hydroxy, alkoxy of 1 to 6 carbon atoms, carboxy, carbalkoxy wherein alkoxy contains 1 to 6 carbon atoms cyano and halo or disubstituted with (CO₂ A)₂ wherein A is hydrogen or alkyl of 1 to 6 carbon atoms.
 10. A compound according to claim 9 wherein R₅ is nitromethyl, hydroxymethyl, alkoxymethyl where alkoxy contains 1 to 6 carbon atoms, carboxymethyl, carbalkoxymethyl, bis(carboxy)methyl, bis(carbalkoxy)methyl, cyanomethyl or halomethyl.
 11. A compound according to claim 1wherein: X is O or S; n has a value of 4 to 8; R₁ is hydrogen, alkyl or aralkyl of up to 12 carbon atoms; R₂ is hydrogen, alkyl of 1 to 4 carbon atoms, or phenyl; R₃ is hydroxy, alkanoyloxy of 1 to 4 carbon atoms or benzyloxy; R₄ is hydrogen, alkyl of 1 to 9 carbon atoms, cycloalkyl of 5 to 8 carbon atoms, phenyl, naphthyl, alkyl of 1 to 6 carbon atoms substituted with phenyl, naphthyl or cycloalkyl of 5 to 8 carbon atoms, any of said phenyl rings or naphthyl rings being unsubstituted or substituted with halo, trifluoromethyl, alkyl of 1 to 6 carbon atoms, alkoxy of 1 to 6 carbon atoms or nitro; R₅ is alkyl of 1 to 6 carbon atoms, phenyl, phenylalkyl wherein alkyl contains 1 to 6 carbon atoms, carboxy or carbalkoxy wherein alkoxy contains up to 12 carbon atoms.
 12. A compound according to claim 11 wherein n is 5, 6 or
 7. 13. A compound according to claim 11 wherein R₃ is hydroxy.
 14. A compound according to claim 11 wherein R₄ is alkyl of 1 to 9 carbon atoms.
 15. A compound according to claim 11 wherein R₅ is alkyl of 1 to 6 carbon atoms.
 16. A compound according to claim 11 wherein:n is 5, 6 or 7; R₂ is hydrogen, methyl, ethyl or phenyl; R₃ is hydroxy; R₄ is hydrogen or alkyl of 1 to 9 carbon atoms; R₅ is alkyl of 1 to 6 carbon atoms, phenyl, phenylalkyl wherein alkyl contains from 1 to 6 carbon atoms, carboxy or carbalkoxy wherein alkoxy contains up to 12 carbon atoms.
 17. A compound according to claim 16 wherein X is O.
 18. A compound according to claim 16 wherein R₁ is hydrogen or alkyl of 1 to 6 carbon atoms.
 19. A compound according to claim 16 wherein R₂ is hydrogen.
 20. A compound according to claim 16 wherein R₂ is methyl.
 21. A compound according to claim 16 wherein R₄ is alkyl of 4 to 9 carbon atoms.
 22. A compound according to claim 21 wherein R₄ is n-pentyl, n-hexyl or n-heptyl.
 23. A compound according to claim 22 wherein R₄ is n-hexyl.
 24. A compound according to claim 21 wherein R₄ is hex-2-yl, hept-2-yl or oct-2-yl.
 25. A compound according to claim 16 wherein R₅ is alkyl of 1 to 6 carbon atoms.
 26. A compound according to claim 25 wherein R₅ is methyl.
 27. The sodium salt of a compound according to claim 16 wherein R₁ is hydrogen.
 28. 1-(3'-Hydroxy-3'-methyl-n-nonyl)-3-methyl-5-(6"-carboxy-n-hexyl)hydantoin.
 29. The compound according to claim 1 which is 1-(3-hydroxy-3-methyl-n-nonyl)-3-cyanomethyl-5-(6-carbomethoxy-n-hexyl)hydantoin.
 30. The sodium salt of the compound of claim
 28. 31. A pharmaceutical composition for effecting a prostaglandin-like response comprising an effective amount of a compound according to claim 1 and a pharmaceutically acceptable carrier.
 32. The method of effecting a prostaglandin-like response in humans and other animals which comprises administering thereto an effective amount of a compound according to claim
 1. 